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Registro completo
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Biblioteca (s) : |
INIA Tacuarembó. |
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Fecha : |
21/02/2014 |
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Actualizado : |
16/08/2017 |
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Tipo de producción científica : |
Tesis |
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Autor : |
DO CANTO, J. |
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Afiliación : |
JAVIER DO CANTO FAGUNDEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Genetic studies on self-fertility in perennial ryegrass (Lolium perenne L.) with implications for hybrid breeding in allogamous grasses: thesis Doctor of Philosophy. |
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Fecha de publicación : |
2017 |
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Fuente / Imprenta : |
Ames, Iowa: Iowa State University, 2017. |
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Páginas : |
140 p. |
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Idioma : |
Inglés |
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Contenido : |
Perennial grasses have diverse uses and are relevant from the agronomic and economic point of view, with main uses as forage, turf and bioenergy. In the grass family polyploidy is prevalent and both autopolyploids and allopolyploids are present. Also, within grasses there are a range of breeding systems, but hermaphrodite flower is the most frequent floral condition. Cross-pollination in species with hermaphrodite flowers is imposed by a gametophytic genetic self-incompatibility (SI). SI is controlled by two multiallelic and independent loci, S and Z. The incompatibility phenotype of the pollen grain is determined by its haploid genome. A pollen grain is incompatible when the same S and Z alleles carried by pollen are present in the pistil. This SI mechanism keeps its functionality at higher ploidy levels. Understanding the mechanisms involved in the breakdown of SI are crucial for implementing novel breeding practices. The aim of this work is to increase the knowledge of SF in outcrossing grasses for the purpose of inbred line development and making hybrid breeding possible. Mutations at S, Z and at a third locus are known to cause self-fertility (SF). In perennial ryegrass (Lolium perenne), a locus conferring SF is located in linkage group 5. Using segregation and linkage analysis, the SF locus region was reduced to 1.6 cM. This locus explained 94% of the observed variability. By aligning the flanking marker sequences to the Brachypodium distachium reference genome, it was found that it corresponds to an 807 Kbp region in B. distachium. This locus was studied at the tetraploid level and it was found that SF remained functional, the SF locus genotype was the main determinant of pollen compatibility explaining 54% of the variation, and there is incomplete dominance between alleles at this locus in the diploid pollen grain. The prospects of migrating the SF locus from perennial ryegrass to other related self-incompatible were discussed. Based on the available information on hybridization between Lolium and Festuca species, different types of crosses were propose according to the particular species involved. The results and observations presented here contribute to a better understanding of the trait at both diploid and tetraploid levels and are promising as SF may readily be incorporated into breeding programs. MenosPerennial grasses have diverse uses and are relevant from the agronomic and economic point of view, with main uses as forage, turf and bioenergy. In the grass family polyploidy is prevalent and both autopolyploids and allopolyploids are present. Also, within grasses there are a range of breeding systems, but hermaphrodite flower is the most frequent floral condition. Cross-pollination in species with hermaphrodite flowers is imposed by a gametophytic genetic self-incompatibility (SI). SI is controlled by two multiallelic and independent loci, S and Z. The incompatibility phenotype of the pollen grain is determined by its haploid genome. A pollen grain is incompatible when the same S and Z alleles carried by pollen are present in the pistil. This SI mechanism keeps its functionality at higher ploidy levels. Understanding the mechanisms involved in the breakdown of SI are crucial for implementing novel breeding practices. The aim of this work is to increase the knowledge of SF in outcrossing grasses for the purpose of inbred line development and making hybrid breeding possible. Mutations at S, Z and at a third locus are known to cause self-fertility (SF). In perennial ryegrass (Lolium perenne), a locus conferring SF is located in linkage group 5. Using segregation and linkage analysis, the SF locus region was reduced to 1.6 cM. This locus explained 94% of the observed variability. By aligning the flanking marker sequences to the Brachypodium distachium reference genome, it was... Presentar Todo |
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Palabras claves : |
PERENNIAL GRASSES. |
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Thesagro : |
GRAMINEAS PERENNES; LOLIUM PERENNE. |
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Asunto categoría : |
A50 Investigación agraria |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/7197/1/CDocuments-and-SettingsachiacchioEscritorioDO-CANTOTHESIS-JAVIER.pdf
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Marc : |
LEADER 02903nam a2200157 a 4500
001 1028590
005 2017-08-16
008 2017 bl uuuu m 00u1 u #d
100 1 $aDO CANTO, J.
245 $aGenetic studies on self-fertility in perennial ryegrass (Lolium perenne L.) with implications for hybrid breeding in allogamous grasses$bthesis Doctor of Philosophy.$h[electronic resource]
260 $aAmes, Iowa: Iowa State University$c2017
300 $a140 p.
520 $aPerennial grasses have diverse uses and are relevant from the agronomic and economic point of view, with main uses as forage, turf and bioenergy. In the grass family polyploidy is prevalent and both autopolyploids and allopolyploids are present. Also, within grasses there are a range of breeding systems, but hermaphrodite flower is the most frequent floral condition. Cross-pollination in species with hermaphrodite flowers is imposed by a gametophytic genetic self-incompatibility (SI). SI is controlled by two multiallelic and independent loci, S and Z. The incompatibility phenotype of the pollen grain is determined by its haploid genome. A pollen grain is incompatible when the same S and Z alleles carried by pollen are present in the pistil. This SI mechanism keeps its functionality at higher ploidy levels. Understanding the mechanisms involved in the breakdown of SI are crucial for implementing novel breeding practices. The aim of this work is to increase the knowledge of SF in outcrossing grasses for the purpose of inbred line development and making hybrid breeding possible. Mutations at S, Z and at a third locus are known to cause self-fertility (SF). In perennial ryegrass (Lolium perenne), a locus conferring SF is located in linkage group 5. Using segregation and linkage analysis, the SF locus region was reduced to 1.6 cM. This locus explained 94% of the observed variability. By aligning the flanking marker sequences to the Brachypodium distachium reference genome, it was found that it corresponds to an 807 Kbp region in B. distachium. This locus was studied at the tetraploid level and it was found that SF remained functional, the SF locus genotype was the main determinant of pollen compatibility explaining 54% of the variation, and there is incomplete dominance between alleles at this locus in the diploid pollen grain. The prospects of migrating the SF locus from perennial ryegrass to other related self-incompatible were discussed. Based on the available information on hybridization between Lolium and Festuca species, different types of crosses were propose according to the particular species involved. The results and observations presented here contribute to a better understanding of the trait at both diploid and tetraploid levels and are promising as SF may readily be incorporated into breeding programs.
650 $aGRAMINEAS PERENNES
650 $aLOLIUM PERENNE
653 $aPERENNIAL GRASSES
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INIA Tacuarembó (TBO) |
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| Registros recuperados : 58 | |
| 9. |  | REYNO, R.; DO CANTO, J.; REAL, D. Germplasm evaluation and frost tolerance improvement of Setaria sphacelata in Uruguay. In: Michalk, D.L; Millar, G.D.; Badgery, W.B.; Broadfoot, K.M.; eds. International Grassland Congress, 22., 15-19 September, Sydney (AT), 2013. Proceedings. Revitalising grasslands to sustain our communities 153-154| Tipo: Trabajos en Congresos/Conferencias |
| Biblioteca(s): INIA Tacuarembó. |
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| 13. | | MARTÍNEZ, S.; ESCALANTE, F.; DO CANTO, J. P3. Patología y selección por resistencia a Pyricularia oryzae en raigrás (Lolium multiflorum). [Poster]. Posters. In: Sociedad Uruguaya de Fitopatología (SUFIT). Jornada Uruguaya de Fitopatología, 7., Jornada Uruguaya de Protección Vegetal, 5., 10 noviembre 2023, Montevideo, Uruguay. Libro de resúmenes. 30 años SUFIT, 1993-2023. Montevideo (UY): Sociedad Uruguay de Fitopatología (SUFIT), 2023. p. 30. Autor correspondencia: e-mail: jdocanto@inia.org.uy| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA Las Brujas. |
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| 18. | | DO CANTO, J.; REAL, D.; REYNO, R.; ALTIER, N. Evaluación de clones de Paspalum notatum en distintos ambientes: producción de forraje y semillas, y reacción frente Claviceps paspali / Evaluation of Paspalum notatum clones in different environments: forage and seed production, and tolerance to Claviceps paspali. [Poster] + [Resumen]. In: JORNADAS DE MEJORAMIENTO GENÉTICO DE FORRAJERAS, 2010, Lavallol, Buenos Aires, AR. [Actas]. [Lavallol, AR: UNLP. Instituto Fitotécnico de Santa Catalina], 2010.| Biblioteca(s): INIA Tacuarembó. |
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| 20. | | DO CANTO, J.; REYNO, R.; REAL, D.; REVELL, C. Seed softening patterns of forage legumes in a temperate/subtropical environment in Uruguay. Chilean Journal of Agricultural Research, 2013, v. 73, no. 1, p. 41-47.DOI: http://dx.doi.org/10.4067/S0718-58392013000100006 Hitory article: Received: 22 May 2012; Accepted: 15 November 2012. ACKNOWLEDGEMENTS: The authors wish to acknowledge the technicians Ana Viana, Ruben Merola, and Mauro Zarza for their assistance in the field and laboratory phases. Funding...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Tacuarembó. |
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| Registros recuperados : 58 | |
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